E-Posters-10068

10068_Lanzer

Pdf Summary

This pilot study compared the effects of systemic and vaginal estrogen on gene expression involved in wound healing in postmenopausal women with pelvic organ prolapse. The study found that the route of estrogen administration had differential effects on genes involved in the wound healing pathway, particularly in inflammation, proliferation, and remodeling. Vaginal estrogen increased expression of genes involved in collagen synthesis to a greater degree than systemic estrogen. Significant correlations were also found between matrix metalloproteinases and their regulators, further validating the results. The study highlights the potential implications of estrogen administration route on the wound healing pathway in postmenopausal women with pelvic organ prolapse.<br /><br />The study included postmenopausal women undergoing surgery for pelvic organ prolapse repair. Biopsies were obtained from three groups: no history of estrogen use in the past 6 months, vaginal estrogen cream use for more than 3 months, and systemic estrogen use for more than 3 months. Quantitative real-time PCR was used to assess genes critical for the wound healing pathway in the vagina. Fold changes in gene expression were calculated using ∆∆CT with housekeeping genes as reference. The results showed significant differences in gene expression between the vaginal and systemic estrogen groups, particularly for genes involved in collagen synthesis.<br /><br />The study suggests that the route of estrogen administration may affect wound healing in vaginal tissue after reconstructive surgery. It also emphasizes the importance of considering the differential effects of estrogen administration routes on genes involved in the wound healing pathway. The findings of this pilot study provide insights into the potential implications of estrogen administration route for postmenopausal women with pelvic organ prolapse. Further research is needed to fully understand the effects of estrogen administration on wound healing in this population.

Keywords

pilot study

systemic estrogen

vaginal estrogen

gene expression

wound healing

postmenopausal women

pelvic organ prolapse

collagen synthesis

estrogen administration route

reconstructive surgery